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Immobilization of an antithrombin–heparin complex on gold: Anticoagulant properties and platelet interactions

Paper ID Volume ID Publish Year Pages File Format Full-Text
1604 87 2011 6 PDF Available
Title
Immobilization of an antithrombin–heparin complex on gold: Anticoagulant properties and platelet interactions
Abstract

The anticoagulant properties and platelet interactions of gold surfaces modified with an antithrombin–heparin (ATH) complex are reported. ATH was attached to gold through either a short disulfide (linker) or a thiol-terminated polyethylene oxide (PEO) (linker, spacer). Analogous surfaces were prepared with uncomplexed heparin. Antithrombin (AT) uptake was measured before and after selectively destroying the active pentasaccharide sequence of the heparin moiety, and was found to be predominantly through the active sequence on all of the surfaces. AT binding was higher on the ATH surfaces than on the corresponding heparin surfaces. Heparin activity was assessed by an anti-factor Xa assay. The ratio of active heparin density (from the anti-FXa assay) to total heparin density was taken as a measure of heparin bioactivity. The ratio was greater on the ATH- than on the heparin-modified surfaces, i.e. the PEO–ATH surfaces showed the greater proportion of active heparin. Platelet adhesion from flowing whole blood was found to be reduced on PEO- and ATH-modified surfaces compared to bare gold. The PEO–ATH modified surfaces, but not the heparinized surfaces, were shown to prolong the clotting time of recalcified plasma.

Keywords
Heparin; Antithrombin; Polyethylene oxide; Anticoagulation; Platelet adhesion
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 5, May 2011, Pages 2029–2034
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us