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Surface characteristics of acrylic modified polysulfone membranes improves renal proximal tubule cell adhesion and spreading

Paper ID Volume ID Publish Year Pages File Format Full-Text
1608 87 2011 10 PDF Available
Title
Surface characteristics of acrylic modified polysulfone membranes improves renal proximal tubule cell adhesion and spreading
Abstract

Current polyvinylpyrrolidone-modified polysulfone (PVP-PSU) membranes in haemodialysers do not facilitate the attachment and proliferation of renal proximal tubule cells (RPTCs). For bioartificial kidney (BAK) development expensive extracellular matrices are employed to ensure the PVP-PSU membranes can serve as a substrate for RPTCs. In this study we modified PSU using an acrylic monomer (am-PSU) and polymerization using ultraviolet irradiation. We demonstrated that on adjusting the PSU or acrylic content of the membranes the wettability and surface chemistry were altered, and this affected the amount of fibronectin (Fn) that was adsorbed onto the membranes. Using an integrin blocking assay we ascertained that Fn is an important extracellular matrix component that mediates RPTC attachment. The amount of Fn adsorbed also led to different bioresponses of RPTCs, which were evaluated using attachment and proliferation assays and qualitative quantification of vinculin, focal adhesion kinase, zonula occludens and Na+/K+ ATPase. Our optimized membrane, am-PSU1 (21.4% C–O groups, 19.1% PVP-PSU; contact angle 71.5–80.80, PVP-PSU: 52.4–67.50), supports a confluent monolayer of RPTCs and prevents creatinine and inulin diffusion from the apical to the basal side, meeting the requirements for application in BAKs. However, further in vivo evaluation to assess the full functionality of RPTCs on am-PSU1 is required.

Keywords
Renal epithelial tissue engineering; Tissue engineering scaffold; ECM adhesion; Scaffold wettability; Haemodialysis
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Surface characteristics of acrylic modified polysulfone membranes improves renal proximal tubule cell adhesion and spreading
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 5, May 2011, Pages 2060–2069
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us