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Tools for genome-wide strain design and construction

Paper ID Volume ID Publish Year Pages File Format Full-Text
16095 42485 2012 6 PDF Available
Tools for genome-wide strain design and construction

Advances in DNA sequencing and synthesis technologies concurrent with the development of new recombinant DNA approaches have enabled the extension of directed evolution algorithms to the genome-scale. It is now possible to simultaneously map the effect of mutation(s) in each and every gene in the genome onto almost any screenable or selectable phenotype in less than a week. Such maps can be used to direct the design and construction of libraries containing billions of rationally designed combinatorial mutations. Such combinatorial libraries can now also be created and evaluated in less than a week. The review presents and discusses these new technologies within the context of directed evolution and inverse metabolic engineering.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (263 K)Download as PowerPoint slideHighlights► Advances in sequencing, nucleotide synthesis and recombinant DNA have enabled faster searching of genomes for target discovery. ► The extension of directed protein evolution algorithms to the engineering of multi-gene phenotypes is discussed.

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Tools for genome-wide strain design and construction
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 23, Issue 5, October 2012, Pages 666–671
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Physical Sciences and Engineering Chemical Engineering Bioengineering