fulltext.study @t Gmail

Recent advances in genetic code engineering in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
16106 42485 2012 7 PDF Available
Title
Recent advances in genetic code engineering in Escherichia coli
Abstract

The expansion of the genetic code is gradually becoming a core discipline in Synthetic Biology. It offers the best possible platform for the transfer of numerous chemical reactions and processes from the chemical synthetic laboratory into the biochemistry of living cells. The incorporation of biologically occurring or chemically synthesized non-canonical amino acids into recombinant proteins and even proteomes via reprogrammed protein translation is in the heart of these efforts. Orthogonal pairs consisting of aminoacyl-tRNA synthetase and its cognate tRNA proved to be a general tool for the assignment of certain codons of the genetic code with a maximum degree of chemical liberty.Here, we highlight recent developments that should provide a solid basis for the development of generalist tools enabling a controlled variation of chemical composition in proteins and even proteomes. This will take place in the frame of a greatly expanded genetic code with emancipated codons liberated from the current function or with totally new coding units.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (171 K)Download as PowerPoint slideHighlights► Highlights incorporation of industrially relevant non-canonical amino acids. ► Multi-labeling of proteins with distinct non-canonical amino acids. ► Recent developments of new orthogonal pairs. ► Release factor 1 knockout strains for amber codon reassignment. ► Codon emancipation for proteome-wide integration of new chemistries into living cells.

First Page Preview
Recent advances in genetic code engineering in Escherichia coli
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 23, Issue 5, October 2012, Pages 751–757
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us