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Bacterial signaling systems as platforms for rational design of new generations of biosensors

Paper ID Volume ID Publish Year Pages File Format Full-Text
16108 42485 2012 7 PDF Available
Title
Bacterial signaling systems as platforms for rational design of new generations of biosensors
Abstract

Bacterial signal-responsive regulatory circuits have been employed as platform to design and construct whole-cell bacterial biosensors for reporting toxicity. A new generation of biosensors with improved performance and a wide application range has emerged after the application of synthetic biology concepts to biosensor design. Site-directed mutagenesis, directed evolution and domain swapping were applied to upgrade signal detection or to create novel sensor modules. Rewiring of the genetic circuits allows improving the determinations and reduces the heterogeneity of the response between individual reporter cells. Moreover, the assembly of natural or engineered modules to biosensor platforms provides innovative outputs, expanding the range of application of these devises, from monitoring toxics and bioremediation to killing targeted cells.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (225 K)Download as PowerPoint slideHighlights► We describe the use of synthetic biology concepts for bacterial biosensor design. ► Rewiring sensor/transducer modules provide improved bioreporter performance. ► Novel outputs can be embedded into existing regulatory circuits. ► Bioremediation, bioenergy and chemotherapy are future application trends.

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Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 23, Issue 5, October 2012, Pages 766–772
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us