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High confidence determination of specific protein–protein interactions using quantitative mass spectrometry

Paper ID Volume ID Publish Year Pages File Format Full-Text
16204 42491 2008 7 PDF Available
Title
High confidence determination of specific protein–protein interactions using quantitative mass spectrometry
Abstract

In recent years, interactions between proteins have successfully been determined by mass spectrometry. A limitation of this technology has been the need for extensive purification, which restricts throughput and implies a tradeoff between specificity and the ability to detect weak or transient interactions. Quantitative proteomics sidesteps this problem by directly comparing specific and control pull-downs. Specific interaction partners are revealed by their quantitative ratios rather than by gel-based visualization and can be retrieved from a vast excess of background proteins. This principle is revolutionizing the protein interaction field as demonstrated by recent applications in fields as diverse as tyrosine signaling pathways, cell adhesion, and chromatin biology.

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High confidence determination of specific protein–protein interactions using quantitative mass spectrometry
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Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 19, Issue 4, August 2008, Pages 331–337
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us