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Poly(β-amino amine) cross-linked PEIs as highly efficient gene vectors

Paper ID Volume ID Publish Year Pages File Format Full-Text
1622 87 2011 9 PDF Available
Title
Poly(β-amino amine) cross-linked PEIs as highly efficient gene vectors
Abstract

To increase the release of DNA into the cytoplasm and further improve transgene expression of nucleic acid novel polymeric gene carriers were prepared which would be biodegradable under the reducing conditions in the cytoplasm. Disulfide-containing poly(β-amino amine)s were first synthesized and then used to cross-link low molecular weight polyethyleneimine (1800 Da) through Michael addition to obtain SS-PBAA-PEIs as the final gene carriers. The physicochemical characteristics of SS-PBAA-PEI/DNA complexes were characterized. In vitro transfection mediated by the SS-PBAA-PEIs under serum conditions was carried out. Cell uptake of the gene delivery systems was observed by confocal laser scanning microscopy. The results of the physicochemical characterisation demonstrated that the SS-PBAA-PEIs could efficiently condense DNA. In vitro transfection under serum conditions showed that SS-PBAA-PEIs had comparable or even higher transfection efficiencies than 25 kDa PEI. And SS-PBAA-PEIs showed much lower cytotoxicity compared with 25 kDa PEI. In summary, the SS-PBAA-PEIs possess great potential as non-viral gene vectors and exhibit high transfection efficiency under serum conditions.

Graphical abstractThe disulfide-contained polymers, SS-PBAA-PEIs were prepared by using disulfide-contained poly(β-amino amine)s to crosslink 1800 Da PEI. It was found that SS-PBAA-PEIs exhibit low toxicity and high transfection efficiency.Figure optionsDownload full-size imageDownload high-quality image (138 K)Download as PowerPoint slide

Keywords
Poly(β-amino amine); Polyethyleneimine; Gene vector; Transfection efficiency; Bioreducible
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Poly(β-amino amine) cross-linked PEIs as highly efficient gene vectors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 5, May 2011, Pages 2200–2208
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us