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Quantitative in vivo microscopy: the return from the ‘omics’

Paper ID Volume ID Publish Year Pages File Format Full-Text
16265 42495 2006 10 PDF Available
Title
Quantitative in vivo microscopy: the return from the ‘omics’
Abstract

The confluence of recent advances in microscopy instrumentation and image analysis, coupled with the widespread use of GFP-like proteins as reporters of gene expression, has opened the door to high-throughput in vivo studies that can provide the morphological and temporal context to the biochemical pathways regulating cell function. We are now able to quantify the concentration and three-dimensional distribution of multiple spectrally resolved GFP-tagged proteins. Using automatic segmentation and tracking we can then measure the dynamics of the processes in which these elements are involved. In this way, parallel studies are feasible where multiple cell colonies treated with drugs or gene expression repressors can be monitored and analyzed to study the dynamics of relevant biological processes.

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Quantitative in vivo microscopy: the return from the ‘omics’
Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 17, Issue 5, October 2006, Pages 501–510
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering