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Microfluidic approaches for systems and synthetic biology

Paper ID Volume ID Publish Year Pages File Format Full-Text
16300 42497 2010 7 PDF Available
Title
Microfluidic approaches for systems and synthetic biology
Abstract

Microfluidic systems miniaturise biological experimentation leading to reduced sample volume, analysis time and cost. Recent innovations have allowed the application of -omics approaches on the microfluidic scale. It is now possible to perform 1.5 million PCR reactions simultaneously, obtain transcriptomic data from as little as 150 cells (as few as 2 transcripts per gene of interest) and perform mass-spectrometric analyses online. For synthetic biology, unit operations have been developed that allow de novo construction of synthetic systems from oligonucleotide synthesis through to high-throughput, high efficiency electroporation of single cells or encapsulation into abiotic chassis enabling the processing of thousands of synthetic organisms per hour. Future directions include a push towards integrating more processes into a single device and replacing off-chip analyses where possible.

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Microfluidic approaches for systems and synthetic biology
Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 21, Issue 4, August 2010, Pages 517–523
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering