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Amelogenin-assisted ex vivo remineralization of human enamel: Effects of supersaturation degree and fluoride concentration

Paper ID Volume ID Publish Year Pages File Format Full-Text
1633 87 2011 10 PDF Available
Title
Amelogenin-assisted ex vivo remineralization of human enamel: Effects of supersaturation degree and fluoride concentration
Abstract

The formation of organized nanocrystals that resemble enamel is crucial for successful enamel remineralization. Calcium, phosphate and fluoride ions, and amelogenin are important ingredients for the formation of organized hydroxyapatite (HAP) crystals in vitro. However, the effects of these remineralization agents on the enamel crystal morphology have not been thoroughly studied. The objective of this study was to investigate the effects of fluoride ions, supersaturation degree and amelogenin on the crystal morphology and organization of ex vivo remineralized human enamel. Extracted third molars were sliced thin and acid-etched to provide the enamel surface for immersion in different remineralization solutions. The crystal morphology and mineral phase of the remineralized enamel surface were analyzed by field emission–scanning electron microscopy, attenuated total reflection–Fourier transformed infrared and X-ray diffraction. The concentration of fluoride and the supersaturation degree of hydroxyapatite had significant effects on the crystal morphology and crystal organization, which varied from plate-like loose crystals to rod-like densely packed nanocrystal arrays. Densely packed arrays of fluoridated hydroxyapatite nanorods were observed under the following conditions: σ(HAP) = 10.2 ± 2.0 with 1.5 ± 0.5 mg l−1 fluoride and 40 ± 10 μg ml−1 amelogenin, pH 6.8 ± 0.4. A phase diagram summarizes the conditions that form dense or loose hydroxyapatite nanocrystal structures. This study provides the basis for the development of novel dental materials for caries management.

Keywords
Supersaturation degree; Enamel-like crystals; Fluoride; Remineralization; Amelogenin
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Amelogenin-assisted ex vivo remineralization of human enamel: Effects of supersaturation degree and fluoride concentration
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 5, May 2011, Pages 2293–2302
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us