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Fast sampling for quantitative microbial metabolomics

Paper ID Volume ID Publish Year Pages File Format Full-Text
16452 42503 2010 8 PDF Available
Title
Fast sampling for quantitative microbial metabolomics
Abstract

Targeted metabolomics, aimed at the study of metabolic reaction networks and their regulation in vivo, is a rapidly emerging field in systems biology. Obtaining proper quantitative snapshots of the microbial metabolome requires fast sampling, immediate quenching of enzymatic activity, separation of exometabolome and endometabolome, complete metabolite extraction from the cells, and reliable high-throughput analysis methods. This review addresses the current state of the art of rapid sampling and quenching for microbial metabolomics. Several robust and reliable rapid sampling devices have been developed. Various quenching and separation procedures have been proposed and applied, but with respect to the reliability of the different methods the literature is contradictory. To date a reliable universal method applicable to different microbial species is still lacking.

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Fast sampling for quantitative microbial metabolomics
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Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 21, Issue 1, February 2010, Pages 27–34
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us