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Engineering next generation proteases

Paper ID Volume ID Publish Year Pages File Format Full-Text
16487 42505 2009 8 PDF Available
Title
Engineering next generation proteases
Abstract

The engineering of novel and precise sequence specificity into proteases will provide an important route to the development of exciting new tools for analytical, biotechnological, and therapeutic applications. Significant progress has been made in reprogramming protease specificity, largely because of the development of high-throughput assay technologies allowing the isolation of protease variants from large libraries. For example, using directed evolution as well as other approaches, proteases have been reprogrammed to cleave substrates containing a variety of amino acids in the P1 and P1′ positions including a post-translationally modified tyrosine, a specificity not yet identified in any naturally occurring protease. Together, these recent advances represent substantial progress that could soon enable the widespread application of engineered proteases.

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Engineering next generation proteases
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Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 20, Issue 4, August 2009, Pages 390–397
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us