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Protein engineering in designing tailored enzymes and microorganisms for biofuels production

Paper ID Volume ID Publish Year Pages File Format Full-Text
16490 42505 2009 8 PDF Available
Title
Protein engineering in designing tailored enzymes and microorganisms for biofuels production
Abstract

Lignocellulosic biofuels represent a sustainable, renewable, and the only foreseeable alternative energy source to transportation fossil fuels. However, the recalcitrant nature of lignocellulose poses technical hurdles to an economically viable biorefinery. Low enzymatic hydrolysis efficiency and low productivity, yield, and titer of biofuels are among the top cost contributors. Protein engineering has been used to improve the performance of lignocellulose-degrading enzymes, as well as proteins involved in biofuel synthesis pathways. Unlike its great success seen in other industrial applications, protein engineering has achieved only modest results in improving the lignocellulose-to-biofuels efficiency. This review will discuss the unique challenges that protein engineering faces in the process of converting lignocellulose to biofuels and how they are addressed by recent advances in this field.

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Protein engineering in designing tailored enzymes and microorganisms for biofuels production
Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 20, Issue 4, August 2009, Pages 412–419
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering