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Designing evolvable libraries using multi-body potentials

Paper ID Volume ID Publish Year Pages File Format Full-Text
16493 42505 2009 10 PDF Available
Title
Designing evolvable libraries using multi-body potentials
Abstract

Novel high-throughput technologies for directed evolution enable experimental coverage of an impressive number of sequences. Nevertheless, the success of such experiments hinges on the initial sequence libraries. Here we consider the computational design of smart focused libraries and review insights from experimental strategies and theoretic advances in modelling their energy landscapes. In library design as in structure prediction, the applied energy function is the key. Current knowledge-based potentials have proven more successful than purely physics-based ones. Here we summarize novel approaches that extend the classical pairwise treatment of residue contacts towards adaptive knowledge-based multi-body potentials. We suggest that minimal sets of probabilistic constraints will lead to much more efficient sampling of permissible conformations and sequence space.

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Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 20, Issue 4, August 2009, Pages 437–446
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us