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Reporter gene imaging of protein–protein interactions in living subjects

Paper ID Volume ID Publish Year Pages File Format Full-Text
16590 42511 2007 7 PDF Available
Title
Reporter gene imaging of protein–protein interactions in living subjects
Abstract

In the past few years there has been a veritable explosion in the field of reporter gene imaging, with the aim of determining the location, duration and extent of gene expression within living subjects. An important application of this approach is the molecular imaging of interacting protein partners, which could pave the way to functional proteomics in living animals and might provide a tool for the whole-body evaluation of new pharmaceuticals targeted to modulate protein–protein interactions. Three general methods are currently available for imaging protein–protein interactions in living subjects using reporter genes: a modified mammalian two-hybrid system, a bioluminescence resonance energy transfer (BRET) system, and split reporter protein complementation and reconstitution strategies. In the future, these innovative approaches are likely to enhance our appreciation of entire biological pathway systems and their pharmacological regulation.

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Reporter gene imaging of protein–protein interactions in living subjects
Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 18, Issue 1, February 2007, Pages 31–37
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering