fulltext.study @t Gmail

Stable-isotope probing of DNA: insights into the function of uncultivated microorganisms from isotopically labeled metagenomes

Paper ID Volume ID Publish Year Pages File Format Full-Text
16651 42516 2006 8 PDF Available
Title
Stable-isotope probing of DNA: insights into the function of uncultivated microorganisms from isotopically labeled metagenomes
Abstract

Stable-isotope probing (SIP) of environmental genomic DNA allows populations in a natural microbial community to be identified through the incorporation of 13C- or 15N-labeled substrates into biomass. The isotopically labeled DNA is retrieved by density gradient centrifugation, and active microorganisms identified by molecular analysis of the DNA. Recent advances have set the stage for DNA-based SIP with higher sensitivities, shorter incubation times, and more relevant substrate concentrations than used previously. The first demonstration of a DNA-SIP-based metagenome analysis has paved the way for hypothesis-driven studies in environmental genomics.

First Page Preview
Stable-isotope probing of DNA: insights into the function of uncultivated microorganisms from isotopically labeled metagenomes
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 17, Issue 1, February 2006, Pages 59–66
Authors
,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us