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Cloning technologies for protein expression and purification

Paper ID Volume ID Publish Year Pages File Format Full-Text
16725 42535 2006 8 PDF Available
Title
Cloning technologies for protein expression and purification
Abstract

Detailed knowledge of the biochemistry and structure of individual proteins is fundamental to biomedical research. To further our understanding, however, proteins need to be purified in sufficient quantities, usually from recombinant sources. Although the sequences of genomes are now produced in automated factories purified proteins are not, because their behavior is much more variable. The construction of plasmids and viruses to overexpress proteins for their purification is often tedious. Alternatives to traditional methods that are faster, easier and more flexible are needed and are becoming available.

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Cloning technologies for protein expression and purification
Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 17, Issue 4, August 2006, Pages 359–366
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering