Mapping protein–protein interactions by mass spectrometry
Mass spectrometry is currently at the forefront of technologies for mapping protein–protein interactions, as it is a highly sensitive technique that enables the rapid identification of proteins from a variety of biological samples. When used in combination with affinity purification and/or chemical cross-linking, whole or targeted protein interaction networks can be elucidated. Several methods have recently been introduced that display increased specificity and a reduced occurrence of false-positives. In the future, information gained from human protein interaction studies could lead to the discovery of novel pathway associations and therapeutic targets.
Journal: Current Opinion in Biotechnology - Volume 17, Issue 4, August 2006, Pages 394–399