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Effect of acid trehalase (ATH) on impaired yeast vacuolar activity

Paper ID Volume ID Publish Year Pages File Format Full-Text
16740 42608 2016 7 PDF Available
Title
Effect of acid trehalase (ATH) on impaired yeast vacuolar activity
Abstract

•Recombinant yeast has constructed using acid trehalase and confirmed overexpression in cell surface and vacuoles.•The growth of ATH overexpressed in yeast cells on trehalose containing medium decreased the vacuolar activity.•Important proteins such as Proteinase A, B and carboxypeptidase Y associated with CPY pathway was confirmed decrease in the quantitative analysis via 2DE assay when using trehalose containing media.

In this study, this protein was overexpressed in yeast cells grown on trehalose-containing medium to assess its impact on yeast vacuolar activity. ATH was confirmed to be located in both cell surface and vacuoles and the overexpression of ATH was observed to decrease vacuolar activity. Therefore, an assumption was suggested to explain this phenomenon as follows: when grown on containing trehalose medium, the ATH localization at cellular periplasm, but not the vacuole, is prioritized to utilize the extracellular trehalose for cell growth. The multivesicular body pathway (MVB pathway) via which ATH is transported into vacuoles is believed to be down-regulated to favor the accumulation of ATH at cell surface area. By extension, other vacuolar proteins travelling through MVB pathway to reach yeast vacuoles likely also suffer the down regulation. It can be concluded that acid trehalase may contribute down regulation of other vacuolar proteins through MVB pathway. This study suggests that it is a potential of acid trehalase (ATH) on impaired activity of yeast vacuolar.

Graphical abstractIn this study, this protein was overexpressed in yeast cells grown on trehalose-containing medium to assess its impact on yeast vacuolar activity. Acid trehalase (ATH) was confirmed to be located in both cell surface and vacuoles and the overexpression of ATH was observed to decrease vacuolar activity. Therefore, an assumption was suggested to explain this phenomenon as follows: when grown on containing trehalose medium, the ATH localization at cellular periplasm, but not the vacuole, is prioritized to utilize the extracellular trehalose for cell growth. The multivesicular body pathway (MVB pathway) via which ATH is transported into vacuoles is believed to be down-regulated to favor the accumulation of ATH at cell surface area. By extension, other vacuolar proteins travelling through MVB pathway to reach yeast vacuoles likely also suffer the down regulation. It can be concluded that acid trehalase may contribute down regulation of other vacuolar proteins through MVB pathway. This study suggests that it is a potential of ATH on impaired activity of yeast vacuolar.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
ATH; Acid trehalase; Trafficking pathway; Vacuole; Lysosome
First Page Preview
Effect of acid trehalase (ATH) on impaired yeast vacuolar activity
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volumes 93–94, November 2016, Pages 44–50
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering