fulltext.study @t Gmail

A magnetite suspension-based washing method for immunoassays using Escherichia coli cells with autodisplayed Z-domains

Paper ID Volume ID Publish Year Pages File Format Full-Text
16753 42610 2016 8 PDF Available
Title
A magnetite suspension-based washing method for immunoassays using Escherichia coli cells with autodisplayed Z-domains
Abstract

•A new washing method for immunoassays was developed by using magnetite suspension.•The washing method was applied to immunoassay based on E. coli with autodisplayed Z-domain.•Washing parameters were optimized for concentration of magnetite, recovery of E.coli, etc.•The immunoassay with new washing method was effectively used in diagnosis of CRP.

Escherichia coli cells with autodisplayed Z-domains have been used for immunoassays of specific target analytes. In this study, a magnetite suspension was used for the washing step in immunoassays of E. coli cells with autodisplayed Z-domains. This approach enhanced the washing conditions for these immunoassays by determining (1) the optimal concentration of the magnetite suspension, (2) the capacity of the magnetite suspension-based washing method to recover E. coli cells, and (3) the level at which the activity of autodisplayed Z-domains is maintained. In immunoassays of C-reactive protein (CRP), the immunoassay incorporating the magnetite suspension-based washing method showed a sensitivity and limit of detection considerably higher than those of the conventional centrifugation-based washing method. The results indicated that immunoassays incorporating the magnetite suspension-based washing method are effective for medical diagnoses based on CRP assay.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Autodisplay; Immunoassay; Magnetites; Washing stepCRP, C-reactive protein; FACS, fluorescence-activated cell sorting; HRP, horseradish peroxidase; IgG, immunoglobulin G; OD, optical density; OD600, optical density at 600 nm
First Page Preview
A magnetite suspension-based washing method for immunoassays using Escherichia coli cells with autodisplayed Z-domains
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 92, October 2016, Pages 1–8
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us