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Trypsin inactivation by latex fabricated gold nanoparticles: A new strategy towards insect control

Paper ID Volume ID Publish Year Pages File Format Full-Text
16755 42610 2016 8 PDF Available
Title
Trypsin inactivation by latex fabricated gold nanoparticles: A new strategy towards insect control
Abstract

•Catalytic potential of trypsin (a vital insect protease) was reduced by bioactive gold nanoparticles (AuNPs).•AuNPs inhibit catalytic activity of trypsin by multiple chemical interactions.•Proposed nanoparticles mediated enzyme inhibition can be further check for its potential in insect/vector control.

Before applying nanotechnologies in biomedical and environmental areas it is advised to study interactions of nanoparticles and other nanomaterials with biomacromolecule present in living system. Moreover there is scarcity of reports on interactions between nanoparticles and biomaterials. In present report a rapid, ecofriendly method of fabricating stable gold nanoparticles (AuNPs) using latex of Jatropha curcas is reported for the first time. AuNPs found to have characteristic absorption maxima centered at 540 nm, multiple irregular shapes with size range from 20 to 50 nm and have crystalline nature. Latex fabricated AuNPs were found to inhibit catalytic potential of trypsin (a vital enzyme responsible for digestion, insecticide resistance and in several disease conditions). The interactions between AuNPs and trypsin were analyzed by UV–vis spectrophotometry and microwave plasma-atomic emission spectrometry which suggests formation of trypsin-AuNPs complex responsible for lowering catalytic activity of trypsin. Transmission electron microscopy, Fourier transform infrared spectroscopy and particle size distribution studies further confirm complex formation between trypsin and AuNPs. Diverse interactions of metal nanoparticles with proteins such as covalent interaction, electrostatic interactions and binding to SH group of amino acid may be the reasons behind inhibition of trypsin activity. In vivo studies on serum of several vectors and agriculturally important pests supported instrumental results on AuNPs induced trypsin inhibition. This work will bring a new research direction to explore eco-friendly nanoparticle in insect control via inhibition of enzyme catalytic potential.

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Keywords
Proteases; Trypsin; Plant latex; Gold nanoparticles; Enzyme inactivation; Insect control
First Page Preview
Trypsin inactivation by latex fabricated gold nanoparticles: A new strategy towards insect control
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 92, October 2016, Pages 18–25
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering