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Why sucrose is the most suitable substrate for pullulan fermentation by Aureobasidium pullulans CGMCC1234?

Paper ID Volume ID Publish Year Pages File Format Full-Text
16759 42610 2016 7 PDF Available
Title
Why sucrose is the most suitable substrate for pullulan fermentation by Aureobasidium pullulans CGMCC1234?
Abstract

•Sucrose was the best substrate for pullulan production by A. pullulans CGMCC1234.•Numerous kestose was produced to reduce the osmotic pressure in early fermentation.•The β-fructofuranosidase activity was strongly dependent on carbon source.•The β-fructofuranosidase production was not directly correlated with the DCW.

This paper studies the metabolic pathway of sucrose in pullulan fermentation by Aureobasidium pullulans. Because of its high pullulan production, sucrose proved to be the best carbon source for pullulan synthesis by A. pullulans CGMCC1234 (36.3 g/L). Compared to other carbon sources, A. pullulans cells reached the stationary phase more quickly in the presence of sucrose. The specific sugar types and concentrations occurring during pullulan fermentation were detected using High Performance Liquid Chromatography (HPLC). HPLC results revealed that sucrose did not simply break down into glucose and fructose in the medium employed. Kestose (22.69 g/L) also accumulated during early stages of fermentation (24 h), which reduced the osmotic pressure of the extracellular fluid and diminished the inhibition of cell growth and pullulan production. β-Fructofuranosidase activity strongly depended on the carbon source. Sucrose was the best inducer of β-fructofuranosidase production. However, β-fructofuranosidase production did not directly and/or proportionally correlate with the growth of A. pullulans cells.

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Keywords
Aureobasidium pullulans; Pullulan; Sucrose; β-Fructofuranosidase
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Why sucrose is the most suitable substrate for pullulan fermentation by Aureobasidium pullulans CGMCC1234?
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 92, October 2016, Pages 49–55
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us