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Physical and chemical immobilization of choline oxidase onto different porous solid supports: Adsorption studies

Paper ID Volume ID Publish Year Pages File Format Full-Text
16776 42611 2016 7 PDF Available
Title
Physical and chemical immobilization of choline oxidase onto different porous solid supports: Adsorption studies
Abstract

•Chemical and physical strategies for the immobilization of choline oxidase onto solid supports.•Promising use as biosensor or bioreactor in continuous flow methodologies.•Immobilized choline oxidase able to be used in analytical purposes.•Freundlich and Langmuir isotherms, kinetic and thermodynamic parameters were tested.•The enzyme activity was monitored by the quenching effect on CdTe quantum dots.

This work carries out for the first time the comparison between the physical and chemical immobilization of choline oxidase onto aminated silica-based porous supports. The influence on the immobilization efficiency of concentration, pH, temperature and contact time between the support and choline oxidase, was evaluated. The immobilization efficiency was estimated taking into consideration the choline oxidase activity, which was assessed by using cadmium telluride (CdTe) quantum dots (QDs), obtained by hydrothermal synthesis, as photoluminescent probes. Hydrogen peroxide produced by enzyme activity was capable of quenching CdTe QDs photoluminescence. The magnitude of the PL quenching process was directly related with the enzyme activity.By comparing the chemical process with the physical adsorption, it was observed that the latter provided the highest choline oxidase immobilization. The equilibrium data were analyzed using Langmuir and Freundlich isotherms and kinetic data were fitted to the pseudo-first-order and pseudo-second-order models. Thermodynamic parameters, such as Gibbs free energy and entropy were also calculated. These results will certainly contribute to the development of new sensing schemes for choline, taking into account the growing demand for its quantification in biological samples.

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Keywords
Choline oxidase; Immobilized enzymes; Adsorption; Kinetic parameters; Biosensors; Enzyme activity
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Physical and chemical immobilization of choline oxidase onto different porous solid supports: Adsorption studies
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 90, August 2016, Pages 76–82
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us