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Ethanol/O2 biofuel cell using a biocathode consisting of laccase/ HOOC-MWCNTs/polydiallyldimethylammonium chloride

Paper ID Volume ID Publish Year Pages File Format Full-Text
16805 42613 2016 7 PDF Available
Title
Ethanol/O2 biofuel cell using a biocathode consisting of laccase/ HOOC-MWCNTs/polydiallyldimethylammonium chloride
Abstract

•PMG could reduce the over potential of NADH oxidation and oxygen reduction.•HOOC-MWCNTs beside PDDA made a suitable microenvironment to preserve the activity of ADH and laccase.•In the optimized condition, BFC produced the power density of 3.98 mW cm−2.

In the present report we focused on the substitution of metallic catalysts by biocatalysts to develop a high efficient biofuel cell. A bioanode and a biocathode were designed using ADH and laccase, respectively. Carboxylated multiwall carbon nanotubes (HOOC-MWCNTs) and polydiallyldimethylammonium chloride (PDDA) were used for immobilizing the enzymes on either polymethylene green (PMG) modified glassy carbon or graphite electrodes. In this way, an ethanol–oxygen biofuel cell was designed in which PDDA/ADH/PDDA/HOOC-MWCNTs/PMG/GC and PDDA/Lac/PDDA/HOOC-MWCNTs/PMG/Gr operated as bioanode and biocathode, respectively. In the optimized condition of O2 saturated PBS (0.1 M, pH 7.5) containing 1 mM ethanol and 1 mM NAD+ the open-circuit voltage reached to a plateau at 504 mV based of which the power density of 3.98 mW cm−2 was obtained.

Keywords
Biofuel cell; Polydiallyldimethylammonium chloride; Carboxylated multiwall carbon nanotube; Laccase; Alcoholdehydrogenase; Polymethylene green
First Page Preview
Ethanol/O2 biofuel cell using a biocathode consisting of laccase/ HOOC-MWCNTs/polydiallyldimethylammonium chloride
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 86, May 2016, Pages 127–133
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering