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CAH1 and CAH2 as key enzymes required for high bicarbonate tolerance of a novel microalga Dunaliella salina HTBS

Paper ID Volume ID Publish Year Pages File Format Full-Text
16811 42614 2016 7 PDF Available
Title
CAH1 and CAH2 as key enzymes required for high bicarbonate tolerance of a novel microalga Dunaliella salina HTBS
Abstract

•An extremely alkalihalophilic microalgae Dunaliella salina HTBS was isolated.•The presence of extracellular CAs (CAH1 and CAH2) in HTBS was confirmed.•CAH1 and CAH2 played a multifunctional role for tolerating high HCO3− concentration.•HTBS featured with tolerance of 70 g L−1 NaHCO3, 30% CO2 and low temperature (7 °C)

Outdoor microalgal cultivation with high concentration bicarbonate has been considered as a strategy for reducing contamination and improving carbon supply efficiency. The mechanism responsible for algae's strong tolerance to high bicarbonate however, remains not clear. In this study, we isolated and characterized a strain and revealed its high bicarbonate tolerant mechanism by analyzing carbonic anhydrase (CA). The strain was identified as Dunaliella salina HTBS with broad temperature adaptability (7–30 °C). The strain grew well under 30% CO2 or 70 g L−1 NaHCO3. In comparison, two periplasm CAs (CAH1 and CAH2) were detected with immunoblotting analysis in HTBS but not in a non-HCO3−—tolerant strain. The finding was also verified by an enzyme inhibition assay in which only HTBS showed significant inhibition by extracellular CA inhibitor. Thus, we inferred that the extracellular CAH1 and CAH2 played a multifunctional role in the toleration of high bicarbonate by HTBS.

Keywords
HTBS, the high tolerance of bicarbonate strain; CA, carbonic anhydrase; CAH1, carbonic anhydrase I; CAH2, carbonic anhydrase II; CAH3, carbonic anhydrase III; CCM, the carbon concentrating mechanism; AZ, acetazolamide; EZ, ethoxyzolamide; SEM, scanning el
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CAH1 and CAH2 as key enzymes required for high bicarbonate tolerance of a novel microalga Dunaliella salina HTBS
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volumes 87–88, June 2016, Pages 17–23
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us