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Improving the synthesis of phenolic polymer using Coprinus cinereus peroxidase mutant Phe230Ala

Paper ID Volume ID Publish Year Pages File Format Full-Text
16813 42614 2016 7 PDF Available
Title
Improving the synthesis of phenolic polymer using Coprinus cinereus peroxidase mutant Phe230Ala
Abstract

•The radical-stable mutant of CiP (F230A) was applied to the phenol polymerization.•The F230A mutant produced a 5.6 fold-higher molecular weight of phenol polymer.•The organic solvents affected the polymerization yield of CiP proteins.•The F230A mutant exhibited lower stability in organic solvents than the wild-type.•The wild-type and F230A mutant showed comparable kinetic parameters.

The F230A mutant of Coprinus cinereus peroxidase (CiP), which has a high stability against radical-inactivation, was previously reported. In the present study, the radical-robust F230A mutant was applied to the oxidative polymerization of phenol. The F230A mutant exhibited better polymerization activities than the wild-type CiP in the presence of water-miscible alcohols i.e., methanol, ethanol, and isopropanol despite its lower stability against alcohols. In particular, the F230A mutant showed a higher consumption of phenol (40%) and yielded phenolic polymer of larger molecular weight (8850 Da) in a 50% (v/v) isopropanol-buffer mixture compared with the wild-type CiP (2% and 1519 Da, respectively). In addition, the wild-type CiP and F230A mutant had no significant differences in enzyme inactivation by physical adsorption on the polymeric products or by heat incubation, and showed comparable kinetic parameters. These results indicate that high radical stability of the F230A mutant and improved solubility of phenolic polymers in alcohol-water cosolvent systems may synergistically contribute to the production of the high molecular weight phenolic polymer.

Keywords
Enzymatic polymerization; Coprinus cinereus; Peroxidase; Radical-robust mutant; Phenol
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Improving the synthesis of phenolic polymer using Coprinus cinereus peroxidase mutant Phe230Ala
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volumes 87–88, June 2016, Pages 37–43
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us