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Metabolic engineering of Corynebacterium glutamicum ATCC13032 to produce S-adenosyl-l-methionine

Paper ID Volume ID Publish Year Pages File Format Full-Text
16833 42616 2016 8 PDF Available
Title
Metabolic engineering of Corynebacterium glutamicum ATCC13032 to produce S-adenosyl-l-methionine
Abstract

•Four genes thrB, metB, mcbR and Ncgl2640 were deleted in C. glutamicum ATCC13032.•Strain HW104 (ATCC13032 ΔthrB ΔmetB ΔmcbR ΔNcg l2640) can accumulate SAM.•Six genes metK, vgb, lysCm, homm, metX and metY were overexpressed in HW104.•HW104/pJYW-4- metK-vgb could accumulate 196.7 mg/L SAM after 48 h cultivation.•SAM production in HW104/pJYW-4- metK-vgb does not need addition of l-methionine.

As an important biological methyl group donor, S-adenosyl-l-methionine is used as nutritional supplement or drug for various diseases, but bacterial strains that can efficiently produce S-adenosyl-l-methionine are not available. In this study, Corynebacterium glutamicum strain HW104 which can accumulate S-adenosyl-l-methionine was constructed from C. glutamicum ATCC13032 by deleting four genes thrB, metB, mcbR and Ncgl2640, and six genes metK, vgb, lysCm, homm, metX and metY were overexpressed in HW104 in different combinations, forming strains HW104/pJYW-4-metK-vgb, HW104/pJYW-4-SAM2C-vgb, HW104/pJYW-4-metK-vgb-metYX, and HW104/pJYW-4-metK-vgb-metYX-homm-lysCm. Fermentation experiments showed that HW104/pJYW-4-metK-vgb produced more S-adenosyl-l-methionine than other strains, and the yield achieved 196.7 mg/L (12.15 mg/g DCW) after 48 h. The results demonstrate the potential application of C. glutamicum for production of S-adenosyl-l-methionine without addition of l-methionine.

Keywords
ATCC13032; S-adenosyl-l-methionine; l-Methionine; Metabolic engineering
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Metabolic engineering of Corynebacterium glutamicum ATCC13032 to produce S-adenosyl-l-methionine
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 83, February 2016, Pages 14–21
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us