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Continuous hydrolysis of carboxymethyl cellulose with cellulase aggregates trapped inside membranes

Paper ID Volume ID Publish Year Pages File Format Full-Text
16846 42617 2015 6 PDF Available
Title
Continuous hydrolysis of carboxymethyl cellulose with cellulase aggregates trapped inside membranes
Abstract

•Cross-linked cellulase aggregate (XCA) was prepared by using a millifluidic reactor.•The XCA can be collected by using a membrane due to its uniform size (350 nm).•Continuous hydrolysis of CMC to reducing sugars is demonstrated.•XCA in membrane shows high enzyme activity and stability.

Enzymatic hydrolysis of cellulose is often conducted in batch processes in which hydrolytic products tend to inhibit enzyme activity. In this study, we report a method for continuous hydrolysis of carboxymethyl cellulose (CMC) by using cross-linked cellulase aggregate (XCA) trapped inside a membrane. XCA particles prepared by using a millifluidic reactor have a uniform size distribution around 350 nm. Because of their large size, XCA particles in solutions can be filtered through a polyethersulfone membrane to collect 87.1 ± 0.9% of XCA particles. The membrane with impregnated XCA can be used as a catalyst for hydrolysis of CMC in a continuous mode. When the CMC concentration is 1.0 g/l and the flow rate is 2 μl/min, 53.9% of CMC is hydrolyzed to reducing sugars. The membrane with XCA is very stable under continuously flowing solutions. After 72 h of reaction, 97.5% of XCA remains inside the membrane.

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Keywords
Cellulase; Membrane; Enzyme aggregate; Enzyme inhibition; Hydrolysis; Bioreactor
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Continuous hydrolysis of carboxymethyl cellulose with cellulase aggregates trapped inside membranes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 78, October 2015, Pages 34–39
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us