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A yeast co-culture-based biosensor for determination of waste water contamination levels

Paper ID Volume ID Publish Year Pages File Format Full-Text
16848 42617 2015 8 PDF Available
Title
A yeast co-culture-based biosensor for determination of waste water contamination levels
Abstract

•Stability of two yeast co-cultures in a BOD sensor was studied.•Substrate specificities of microbial co-culture-based receptor elements were assessed.•New polymer for immobilization of NVP-modified PVA improved the characteristics.•The co-culture of three strains provided for biosensor’s higher analytical signals.

Artificial microbial co-cultures were formed to develop the receptor element of a biosensor for assessment of biological oxygen demand (BOD). The co-cultures possessed broad substrate specificities and enabled assays of water and fermentation products within a broad BOD range (2.4–80 mg/dm3) with a high correlation to the standard method (R = 0.9988). The use of the co-cultures of the yeasts Pichia angusta, Arxula adeninivorans and Debaryomyces hansenii immobilized in N-vinylpyrrolidone-modified poly(vinyl alcohol) enabled developing a BOD biosensor possessing the characteristics not inferior to those in the known biosensors. The results are indicative of a potential of using these co-cultures as the receptor element base in prototype models of instruments for broad application.

Keywords
Co-culture; Bacteria; Yeasts; Biochemical oxygen demand (BOD); Biosensor analyzer; Growth curves
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A yeast co-culture-based biosensor for determination of waste water contamination levels
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 78, October 2015, Pages 46–53
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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