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Quantitative detection of selenate-reducing bacteria by real-time PCR targeting the selenate reductase gene

Paper ID Volume ID Publish Year Pages File Format Full-Text
16866 42619 2016 6 PDF Available
Title
Quantitative detection of selenate-reducing bacteria by real-time PCR targeting the selenate reductase gene
Abstract

•Designed a primer set to target the selenate reductase (SerA).•The primer set has high specificity and positively amplified environmental selenate reducing bacteria.

We designed a primer set to target selenate reductase (SerA) for detecting selenate reducing bacteria (SeRB). Our serA gene-based PCR primer set has high specificity in that it and positively amplified some SeRB, but not denitrifying bacteria (DB). Phylogenetic analysis of serA clone sequences of environmental samples from selenate-reducing membrane biofilm reactor (MBfR) biofilms showed that these sequences were closely grouped and had high similarity to selenate reductase gene sequences from SeRB Thauera selenatis and DB Dechloromonas; however, they were distant to other genes from dimethylsulfoxide (DMSO) enzyme family. Constructing a standard curve targeting the serA gene, we found that the good linearity for the qPCR assay when applied it to quantify SeRB in MBfR biofilms, and the gene copies of SeRB correlated well to the selenate removal percentages. Our results demonstrated the feasibility of using the serA gene-based PCR primer set to detect and quantify SeRB in environmental samples.

Keywords
Selenate reductase; Primer design; DMSO; Membrane biofilm reactor
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Quantitative detection of selenate-reducing bacteria by real-time PCR targeting the selenate reductase gene
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 85, April 2016, Pages 19–24
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us