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Metabolic networks to generate pyruvate, PEP and ATP from glycerol in Pseudomonas fluorescens

Paper ID Volume ID Publish Year Pages File Format Full-Text
16871 42619 2016 6 PDF Available
Title
Metabolic networks to generate pyruvate, PEP and ATP from glycerol in Pseudomonas fluorescens
Abstract

•Pseudomonas fluorescens can grow in mineral medium with 10% glycerol.•H2O2 triggers a metabolic shift resulting in the enhanced production of ATP, PEP and pyruvate.•The enhanced activities of enzymes like PEPC, PEPS and PC are critical to this process.•Intact cells generate pyruvate while cell-free extract synthesizes ATP and PEP.•This metabolic network may be tailored to the synthesis of these valued-added products from glycerol.

Glycerol is a major by-product of the biodiesel industry. In this study we report on the metabolic networks involved in its transformation into pyruvate, phosphoenolpyruvate (PEP) and ATP. When the nutritionally-versatile Pseudomonas fluorescens was exposed to hydrogen peroxide (H2O2) in a mineral medium with glycerol as the sole carbon source, the microbe reconfigured its metabolism to generate adenosine triphosphate (ATP) primarily via substrate-level phosphorylation (SLP). This alternative ATP-producing stratagem resulted in the synthesis of copious amounts of PEP and pyruvate. The production of these metabolites was mediated via the enhanced activities of such enzymes as pyruvate carboxylase (PC) and phosphoenolpyruvate carboxylase (PEPC). The high energy PEP was subsequently converted into ATP with the aid of pyruvate phosphate dikinase (PPDK), phosphoenolpyruvate synthase (PEPS) and pyruvate kinase (PK) with the concomitant formation of pyruvate. The participation of the phospho-transfer enzymes like adenylate kinase (AK) and acetate kinase (ACK) ensured the efficiency of this O2-independent energy-generating machinery. The increased activity of glycerol dehydrogenase (GDH) in the stressed bacteria provided the necessary precursors to fuel this process. This H2O2-induced anaerobic life-style fortuitously evokes metabolic networks to an effective pathway that can be harnessed into the synthesis of ATP, PEP and pyruvate. The bioconversion of glycerol to pyruvate will offer interesting economic benefit.

Keywords
GDH, glycerol dehydrogenase; AK, adenylate kinase; ACK, acetate kinase; CFE, cell free extract; G6PDH, glucose 6-phosphate dehydrogenase; H2O2, hydrogen peroxide; ICDH, isocitrate dehydrogenase; ME, malic enzyme; OP, oxidative phosphorylation; PC, pyruvat
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Metabolic networks to generate pyruvate, PEP and ATP from glycerol in Pseudomonas fluorescens
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 85, April 2016, Pages 51–56
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us