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Synthesis of octyl-β-d-glucopyranoside catalyzed by Thai rosewood β-glucosidase-displaying Pichia pastoris in an aqueous/organic two-phase system

Paper ID Volume ID Publish Year Pages File Format Full-Text
16876 42619 2016 8 PDF Available
Title
Synthesis of octyl-β-d-glucopyranoside catalyzed by Thai rosewood β-glucosidase-displaying Pichia pastoris in an aqueous/organic two-phase system
Abstract

•Thai rosewood β-glucosidase yeast whole cells were used to synthesize octyl-glu.•The efficiency of transglycosylation is much higher than reverse hydrolysis.•Butyl-glu was used as the substrate in transglycosylation reaction.•51.1% and 47.5% conversion rates were achieved in 2 ml and 500 ml reaction systems.•The reuse experiments showed Pp-DCBGL had a good operating stability.

We explored the ability of a Thai rosewood β-glucosidase-displaying P. pastoris whole-cell biocatalyst (Pp-DCBGL) system to synthesize alkyl β-d-glucosides. The primary investigation centered on the synthesis of octyl-β-d-glucopyranoside (octyl-glu, OG). OG could be synthesized through reverse hydrolysis reaction with very low efficiency. Then, OG was synthesized between BG and octanol by a transglycosylation reaction. In a 2-ml reaction system, OG was synthesized with a conversion rate of 51.1% in 3 h when 5 mg/ml BG was utilized as the glucosyl donor under optimized conditions. And, even after being reused four times, the Pp-DCBGL was relatively stable. Additionally, a 500-ml-scale reaction system was conducted in a 2-L stirred reactor with a conversion rate of 47.5% in 1.5 h. Moreover, the conversion rate did not decrease after the whole-cell catalyst was reused two times. In conclusion, Pp-DCBGL has high reaction efficiency and operational stability, which is a powerful biocatalyst available for industrial synthesis.

Keywords
Thai rosewood β-glucosidase; Yeast whole-cell catalyst; Transglycosylation; Octyl-β-d-glucopyranoside; Scale synthesis
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Synthesis of octyl-β-d-glucopyranoside catalyzed by Thai rosewood β-glucosidase-displaying Pichia pastoris in an aqueous/organic two-phase system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 85, April 2016, Pages 90–97
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us