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An efficient ribitol-specific dehydrogenase from Enterobacter aerogenes

Paper ID Volume ID Publish Year Pages File Format Full-Text
16885 42620 2015 9 PDF Available
Title
An efficient ribitol-specific dehydrogenase from Enterobacter aerogenes
Abstract

•It reports a highly active ribitol dehydrogenase (EaRDH) from Enterobacter aerogenes.•Among various polyols, EaRDH exhibits activity only toward ribitol.•EaRDH shows the highest catalytic efficiency among all characterized RDHs.•Docking analyses shed light on the molecular basis of its unusually high activity.

An NAD+-dependent ribitol dehydrogenase from Enterobacter aerogenes KCTC 2190 (EaRDH) was cloned and successfully expressed in Escherichia coli. The complete 729-bp gene was amplified, cloned, expressed, and subsequently purified in an active soluble form using nickel affinity chromatography. The enzyme had an optimal pH and temperature of 11.0 and 45 °C, respectively. Among various polyols, EaRDH exhibited activity only toward ribitol, with Km, Vmax, and kcat/Km values of 10.3 mM, 185 U mg−1, and 30.9 s−1 mM−1, respectively. The enzyme showed strong preference for NAD+ and displayed no detectable activity with NADP+. Homology modeling and sequence analysis of EaRDH, along with its biochemical properties, confirmed that EaRDH belongs to the family of NAD+-dependent ribitol dehydrogenases, a member of short-chain dehydrogenase/reductase (SCOR) family. EaRDH showed the highest activity and unique substrate specificity among all known RDHs. Homology modeling and docking analysis shed light on the molecular basis of its unusually high activity and substrate specificity.

Keywords
Short-chain dehydrogenase/reductase; Enterobacter aerogenes; Ribitol dehydrogenase; Homology modeling
First Page Preview
An efficient ribitol-specific dehydrogenase from Enterobacter aerogenes
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 72, May 2015, Pages 56–64
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering