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AcsA–AcsB: The core of the cellulose synthase complex from Gluconacetobacter hansenii ATCC23769

Paper ID Volume ID Publish Year Pages File Format Full-Text
16896 42621 2016 8 PDF Available
Title
AcsA–AcsB: The core of the cellulose synthase complex from Gluconacetobacter hansenii ATCC23769
Abstract

•Purification of cellulose synthase from Gluconacetobacter hansenii by two methods.•Identification of the post-translational processing site of cellulose synthase.•Determination of the steady state kinetic parameters for cellulose synthase.•Exploration of the role of accessory proteins AcsC, AcsD, and ccpAx.

The gram-negative bacterium, Gluconacetobacter hansenii, produces cellulose of exceptionally high crystallinity in comparison to the cellulose of higher plants. This bacterial cellulose is synthesized and extruded into the extracellular medium by the cellulose synthase complex (CSC). The catalytic component of this complex is encoded by the gene AcsAB. However, several other genes are known to encode proteins critical to cellulose synthesis and are likely components of the bacterial CSC. We have purified an active heterodimer AcsA–AcsB from G. hansenii ATCC23769 to homogeneity by two different methods. With the purified protein, we have determined how it is post-translationally processed, forming the active heterodimer AcsA–AcsB. Additionally, we have performed steady-state kinetic studies on the AcsA–AcsB complex. Finally through mutagenesis studies, we have explored the roles of the postulated CSC proteins AcsC, AcsD, and CcpAx.

Keywords
CSC, cellulose synthase complex; BC, bacterial cellulose; AcsA–AcsB, the active G. hansenii cellulose synthase heterodimerCellulose; Gluconacetobacter hansenii; Cell Wall; Acetobacter; Glycosyltransferase
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AcsA–AcsB: The core of the cellulose synthase complex from Gluconacetobacter hansenii ATCC23769
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 82, January 2016, Pages 58–65
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us