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Influence of immobilization strategies on biosensing response characteristics: A comparative study

Paper ID Volume ID Publish Year Pages File Format Full-Text
16908 42621 2016 7 PDF Available
Title
Influence of immobilization strategies on biosensing response characteristics: A comparative study
Abstract

•A comparison of three immobilization techniques has been made.•Dependence of immobilization technique on biosensing response observed.•Stable conformational orientation of enzyme perceived in covalent immobilization.•Covalent immobilization proves to be the best owing to its zero length.

The immobilization technique plays an important role in fabrication of a biosensor. NiO based cholesterol biosensor has been used to study the effect of various immobilization techniques on the biosensing response characteristics. The biosensors were fabricated by immobilizing cholesterol oxidase on NiO thin films by three different immobilization techniques viz. physisorption, cross-linking and covalent binding. The study reveals a strong dependence of biosensing response on corresponding immobilization technique. The biosensor based on immobilization by covalent bonding shows superior response characteristics as compared to others owing to its zero length. The results highlight the significance of immobilization technique for biosensor fabrication.

Keywords
Biosensor; Immobilization technique; Physisorption; Covalent immobilization; Crosslinking
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Influence of immobilization strategies on biosensing response characteristics: A comparative study
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 82, January 2016, Pages 144–150
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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