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Study of microwave effects on the lipase-catalyzed hydrolysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
16911 42621 2016 9 PDF Available
Title
Study of microwave effects on the lipase-catalyzed hydrolysis
Abstract

•Quantitative mass spectrometry was used to analyze the lipase-catalyzed hydrolysis of triolein.•Hydrolysis reactions performed at various conditions were examined.•The kinetic data provide evidence of the thermal effect of microwave.•Conventional heating at high temperatures (e.g., 50 °C) can be also used to accelerate the hydrolysis reactions.

The effect of microwave heating on lipase-catalyzed reaction remains controversial. It is not clear whether the reaction rate enhancements are purely due to thermal/heating effects or to non-thermal effects. Therefore, quantitative mass spectrometry was used to conduct accurate kinetic analysis of lipase-catalyzed hydrolysis of triolein by microwave and conventional heating. Commercial lipases from Candida rugosa (CRL), Porcine Pancreas (PPL), and Burkholderia cepacia (BCL) were used. Hydrolysis reactions were performed at various temperatures and pH levels, along with various amounts of buffer and enzymes. Hydrolysis product yields at each time point using an internal-standard method showed no significant difference between microwave and conventional heating conditions when the reaction was carried out at the same temperature. CRL showed optimum catalytic activity at 37 °C, while PPL and BCL had better activities at 50 °C. The phosphate buffer was found to give a better hydrolysis yield than the Tris–HCl buffer. Overall results prove that a non-thermal effect does not exist in microwave-assisted lipase hydrolysis of triolein. Therefore, conventional heating at high temperatures (e.g., 50 °C) can be also used to accelerate hydrolysis reactions.

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Keywords
Lipase-catalyzed reaction; Microwave; Mass spectrometry; Triolein; Quantitative analysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 82, January 2016, Pages 164–172
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us