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Lipase in biphasic alginate beads as a biocatalyst for esterification of butyric acid and butanol in aqueous media

Paper ID Volume ID Publish Year Pages File Format Full-Text
16912 42621 2016 7 PDF Available
Title
Lipase in biphasic alginate beads as a biocatalyst for esterification of butyric acid and butanol in aqueous media
Abstract

•Lipase is entrapped in biphasic alginate bead containing a distinct organic phase.•The biphasic beads are able to catalyze esterification in aqueous media.•Organic-aqueous interfacial area inside the bead is maximized by emulsification.•Biphasic beads can be applied in simultaneous fermentation and esterification.•The beads are benign even though the entrapped solvent is toxic to bacteria.

Esterification of organic acids and alcohols in aqueous media is very inefficient due to thermodynamic constraints. However, fermentation processes used to produce organic acids and alcohols are often conducted in aqueous media. To produce esters in aqueous media, biphasic alginate beads with immobilized lipase are developed for in situ esterification of butanol and butyric acid. The biphasic beads contain a solid matrix of calcium alginate and hexadecane together with 5 mg/mL of lipase as the biocatalyst. Hexadecane in the biphasic beads serves as an organic phase to facilitate the esterification reaction. Under optimized conditions, the beads are able to catalyze the production of 0.16 mmol of butyl butyrate from 0.5 mmol of butyric acid and 1.5 mmol of butanol. In contrast, when monophasic beads (without hexadecane) are used, only trace amount of butyl butyrate is produced. One main application of biphasic beads is in simultaneous fermentation and esterification (SFE) because the organic phase inside the beads is very stable and does not leach out into the culture medium. SFE is successfully conducted with an esterification yield of 6.32% using biphasic beads containing iso-octane even though the solvent is proven toxic to the butanol-producing Clostridium spp.

Keywords
Esterification; Butyl butyrate; Lipase; Calcium alginate bead; Biofuel
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Lipase in biphasic alginate beads as a biocatalyst for esterification of butyric acid and butanol in aqueous media
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 82, January 2016, Pages 173–179
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us