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Highly effective detection of inflamed cells using a modified bradykinin ligand labeled with FITC fluorescence

Paper ID Volume ID Publish Year Pages File Format Full-Text
16914 42621 2016 6 PDF Available
Title
Highly effective detection of inflamed cells using a modified bradykinin ligand labeled with FITC fluorescence
Abstract

•Bradykinin (BK) or modified BK (mBK) was used for cell inflammation imaging.•BK-or mBK-based imaging methods are more effective than conventional methods.•The imaging method was also effective for flow-based inflammation analysis of live cells on a slide chip.

Detection of inflammation in live cells is important because long-lasting inflammation is considered to be a primary cause of several diseases. However, few reports have been published on imaging analysis of inflammation in live cells. In this study, we developed an effective imaging system for detection of inflamed cells using a bradykinin ligand (BK) or a modified BK (mBK), which has specific affinity with the cellular B1R receptor. Synthetic BK or mBK labeled with FITC at the N-terminus was employed for discriminating between inflamed and normal cells; this method was found to be effective for detection of inflammation in live cells. In addition, using the mBK-based cell imaging system, we successfully performed flow-based analysis of live cell inflammation on a micro-chip channel, composed of a Starna flow cell and PDMS (Polydimethylsiloxane) walls. The BK-based cell imaging methods designed here would be a useful platform for development of a high-throughput live cell analysis system for investigating the factors underlying inflammation or for screening of anti-inflammation candidate drugs.

Keywords
Inflammation; Bradykinin; Live cell imaging; Flow-based cell analysis
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Highly effective detection of inflamed cells using a modified bradykinin ligand labeled with FITC fluorescence
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 82, January 2016, Pages 191–196
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us