fulltext.study @t Gmail

The influence of budA deletion on glucose metabolism related in 2,3-butanediol production by Klebsiella pneumoniae

Paper ID Volume ID Publish Year Pages File Format Full-Text
16917 42622 2015 8 PDF Available
Title
The influence of budA deletion on glucose metabolism related in 2,3-butanediol production by Klebsiella pneumoniae
Abstract

•We attempted to understand the effects of blocked 2,3-BDO pathway on K. pneumoniae.•We constructed K. pneumoniaeΔwabGΔbudA.•We analyzed carbon flux distribution, transcriptome and extracellular amino acid concentration.•This study provides an insight of K. pneumoniae glucose metabolism.

Klebsiella pneumoniae (K. pneumoniae), which is a promising microorganism for industrial bulk production of 2,3-butanediol (2,3-BDO), naturally converts glucose to 2,3-BDO. The 2,3-BDO biosynthesis from glucose is composed of three steps; α-acetolactate biosynthesis by α-acetolactate synthase (budB); acetoin biosynthesis by α-acetolactate decarboxylase (budA); and 2,3-BDO biosynthesis by acetoin reductase (budC). In an effort to understand the influence of blocked 2,3-BDO pathway on K. pneumoniae glucose metabolism by budA deletion, we constructed K. pneumoniaeΔwabGΔbudA (SGSB106). Carbon flux distribution analysis, transcriptome analysis and extracellular amino acid concentration analysis were carried out to understand the effects of the budA deletion, and K. pneumoniaeΔwabG (SGSB100) was used as a control strain. Approximately 50.3% decrease in CO2 emission; and approximately 3.8-fold increase in amino acid production was observed in SGSB106. In addition to, among the amino acids, valine production significantly increased, suggesting that the branched-chain amino acid biosynthesis (BACC) in SGSB106 was activated by deletion of budA. Furthermore, whole genome transcriptome analysis of SGSB106 and SGSB100, correlates with the results from carbon distribution and amino acids concentration analyses.

Keywords
2 ;3-butanediol; α-acetolactate decarboxylase; Transcriptome analysis; Metabolome analysis; Carbon distribution analysis; Amino acid analysis
First Page Preview
The influence of budA deletion on glucose metabolism related in 2,3-butanediol production by Klebsiella pneumoniae
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volumes 73–74, June 2015, Pages 1–8
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us