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Fungal demethylation of Kraft lignin

Paper ID Volume ID Publish Year Pages File Format Full-Text
16922 42622 2015 7 PDF Available
Title
Fungal demethylation of Kraft lignin
Abstract

•Selected ion flow tube mass spectrometry is used to study metabolism of lignin.•Fungi not degrading lignin are selected by ultrafiltration of the metabolized lignin.•Lignin demethylation by fungi is characterized by Ti-NTA assay.•Demethylated Kraft lignin is obtained on a small batch culture basis.•Lignin fragments noted by HPLC demonstrate various fungal metabolic pathways.

Demethylation of industrial lignin has been for long coveted as a pathway to the production of an abundant natural substitute for fossil-oil derived phenol. In an attempt to possibly identify a novel Kraft lignin-demethylating enzyme, we surveyed a collection of fungi by using selected ion flow tube-mass spectrometry (SIFT-MS). This method readily identifies methanol resulting from lignin demethylation activity. Absidia cylindrospora, and unidentified Cylindrocladium sp. and Aspergillus sp. were shown to metabolize lignin via different pathways, based on the HPLC analysis of lignin fragments. Of these three, Cylindrocladium and Aspergillus were shown to retain most of the lignin intact after 3 weeks in culture, while removing about 40% of the available methoxy groups. Our results demonstrate that after optimization of culture and lignin recovery methods, biological modification of Kraft lignin may be a feasible pathway to obtaining demethylated lignin for further industrial use.

Keywords
SIFT-MS, Selected Ion Flow Tube-Mass Spectrometry; NTA, nitrilo acetic acidLignin demethylation; Fungal culture; Methanol mass spectrometry; Vicinal hydroxyl assay; HPLC
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Fungal demethylation of Kraft lignin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volumes 73–74, June 2015, Pages 44–50
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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