fulltext.study @t Gmail

Improving the catalytic properties of immobilized Lecitase via physical coating with ionic polymers

Paper ID Volume ID Publish Year Pages File Format Full-Text
16928 42623 2014 8 PDF Available
Title
Improving the catalytic properties of immobilized Lecitase via physical coating with ionic polymers
Abstract

•Covalent and adsorbed immobilization of Lecitase Ultra yielded very different activity recoveries.•Activity, stability and specificity of Lecitase are altered (improved in some cases) after coating with ionic polymers.•The effect of the coating strongly depends on the immobilization protocol, experimental conditions and substrate.•Irreversible inhibition rate suggested that there is no stabilization of the open form of the Lecitase.

Lecitase Ultra has been immobilized on cyanogen bromide agarose (via covalent attachment) and on octyl agarose (via physical adsorption on the hydrophobic support by interfacial activation). Both immobilized preparations have been incubated in dextran sulfate (DS) or polyethylenimine (PEI) solutions to coat the enzyme surface. Then, the activity versus different substrates and under different experimental conditions was evaluated. The PEI coating generally produced a significant increase in enzyme activity, in some cases even by more than a 30-fold factor (using the octyl-Lecitase at pH 5 in the hydrolysis of methyl phenyl acetate). In opposition, the DS coating usually produced some negative effects on the enzyme activity. The rate of irreversible inhibition of the covalent preparation using diethyl p-nitrophenylphosphate did not increase after PEI coating suggesting that the increase in Lecitase activity is not a consequence of the stabilization of the open form of Lecitase. Moreover, the coating greatly increased the stability of the immobilized Lecitase, for example using DS and the covalent preparation, the half-life was increased by a 30-fold factor in 30% acetonitrile. The stabilizing effect was not found in all cases, in certain cases even a certain destabilization is found (e.g., octyl-Lecitase-DS at pH 7). Thus, the effects of the ionic polymer coating strongly depend on the substrate, experimental conditions and immobilization technique employed.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Lecitase Ultra; Interfacial activation; Enzyme hyperactivation; Enzyme stabilization; Sulfate dextran; Polyethylenimine
First Page Preview
Improving the catalytic properties of immobilized Lecitase via physical coating with ionic polymers
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 60, 10 June 2014, Pages 1–8
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us