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Immobilization of penicillin G acylase on paramagnetic aldehyde-functionalized mesostructured cellular foams

Paper ID Volume ID Publish Year Pages File Format Full-Text
16932 42623 2014 8 PDF Available
Title
Immobilization of penicillin G acylase on paramagnetic aldehyde-functionalized mesostructured cellular foams
Abstract

•NH2-Fe3O4 nanoparticles were successfully grafted on the outer surface of AMCFs.•PGA molecules were mainly immobilized covalently on the inner surface of PAMCFs.•PGA/PAMCFs show higher initial activity and operational stability.•PGA/PAMCFs are easily recycled by the magnetic field.

Paramagnetic aldehyde-functionalized mesostructured cellular foams (PAMCFs), synthesized by grafting 3-aminopropyltriethoxysilane modified Fe3O4 (NH2-Fe3O4) nanoparticles with larger particle size than the window pore size of MCFs on the outer surface of aldehyde-functionalized mesostructured cellular foams (AMCFs), were investigated as efficient supports for immobilization of penicillin G acylase (PGA). The results show that NH2-Fe3O4 nanoparticles were successfully grafted on the outer surface of AMCFs and PGA molecules were mainly immobilized covalently on the inner surface of PAMCFs, which was because amino groups of NH2-Fe3O4 nanoparticles or PGA molecules reacted with aldehyde groups of AMCFs or PAMCFs to form imine bonds. PGA/PAMCFs-15 showed a rather high initial activity of 9563 U g−1 and retained 89.1% of its initial activity after recycled for 10 times. PGA/PAMCFs are easily recycled by magnetic field in order to replace tedious separation of high-speed centrifugation for mesoporous materials.

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Keywords
Mesostructured cellular foams; Paramagnetic; Aldehyde groups; Penicillin G acylase; Immobilization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 60, 10 June 2014, Pages 32–39
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us