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Dry entrapment of enzymes by epoxy or polyester resins hardened on different solid supports

Paper ID Volume ID Publish Year Pages File Format Full-Text
16934 42623 2014 9 PDF Available
Title
Dry entrapment of enzymes by epoxy or polyester resins hardened on different solid supports
Abstract

•A dry entrapment immobilization method for enzymes was evolved.•Therefore water-free enzyme preparations were embedded in two different polymeric glues.•The method was proofed to be applicable on two very different enzymes, a lipase and a threonine aldolase.

Embedding of enzymes was performed with epoxy or polyester resin by mixing in a dried enzyme preparation before polymerization was started. This fast and low-cost immobilization method produced enzymatically active layers on different solid supports.As model enzymes the well-characterized Thermomyces lanuginosus lipase and a new threonine aldolase from Ashbya gossypii were used. It was shown that T. lanuginosus lipase recombinantly expressed in Aspergillus oryzae is a monomeric enzyme with a molecular mass of 34 kDa, while A. gossypii threonine aldolase expressed in Escherichia coli is a pyridoxal-5′-phosphate binding homotetramer with a mass of 180 kDa.The enzymes were used freeze dried, in four different preparations: freely diffusing, adsorbed on octyl sepharose, as well as cross-linked enzyme aggregates or as suspensions in organic solvent. They were mixed with standard two-component resins and prepared as layers on solid supports made of different materials e.g. metal, glass, polyester. Polymerization led to encapsulated enzyme preparations showing activities comparable to literature values.

Keywords
Immobilization; Epoxy resin; Polyester resin; Thermomyces lanuginosus lipase; Ashbya gossypii threonine aldolase; Cross linked enzyme aggregates
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Dry entrapment of enzymes by epoxy or polyester resins hardened on different solid supports
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 60, 10 June 2014, Pages 47–55
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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