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Fabricating polystyrene fiber-dehydrogenase assemble as a functional biocatalyst

Paper ID Volume ID Publish Year Pages File Format Full-Text
16964 42627 2015 8 PDF Available
Title
Fabricating polystyrene fiber-dehydrogenase assemble as a functional biocatalyst
Abstract

•We fabricated and modified electrospun polystyrene fibers scaffold for biocatalysis.•We characterized the structures and properties of modified electrospun polystyrene fibers.•We evaluate the enzyme loading efficiency, activity and storage stability of ADH.•Nitration of nanofiber can offer acceptable modification for enzyme immobilization.•Electrospun polystyrene fiber-enzyme assembles can be used for bioconversion of CO2 to biofuel.

Immobilization of the enzymes on nano-structured materials is a promising approach to enhance enzyme stabilization, activation and reusability. This study aimed to develop polystyrene fiber-enzyme assembles to catalyze model formaldehyde to methanol dehydrogenation reaction, which is an essential step for bioconversion of CO2 to a renewable bioenergy. We fabricated and modified electrospun polystyrene fibers, which showed high capability to immobilize dehydrogenase for the fiber-enzyme assembles. Results from evaluation of biochemical activities of the fiber-enzyme assemble showed that nitriation with the nitric/sulfuric acid ratio (v/v, 10:1) and silanization treatment delivered desirable enzyme activity and long-term storage stability, showing great promising toward future large-scale applications.

Graphical abstractPolystyrene nanofiber-enzyme assembles were developed to catalyze model formaldehyde to methanol dehydrogenation reaction, which is an essential step for bioconversion of CO2 to a renewable bioenergy. Electrospun polystyrene fibers modified by low ratio of HNO3/H2SO4 offer high enzyme loading efficiency and reusability. Results showed great an implication of nanostructure-enzyme assembles in future biocatalysts.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Biocatalysis; Electrospun polystyrene fiber; Nitration; Enzyme immobilization
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Fabricating polystyrene fiber-dehydrogenase assemble as a functional biocatalyst
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 68, January 2015, Pages 15–22
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us