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A new generation of flowerlike horseradish peroxides as a nanobiocatalyst for superior enzymatic activity

Paper ID Volume ID Publish Year Pages File Format Full-Text
16976 42628 2015 5 PDF Available
Title
A new generation of flowerlike horseradish peroxides as a nanobiocatalyst for superior enzymatic activity
Abstract

•Synthesis of Fe2+ incorporated HRP enzyme hybrid nanoflowers.•Studies of effect of storage temperatures on catalytic activity and stability of hybrid nanoflowers.•Activity measurement of hybrid nanoflowers.

Although various supports including nanomaterials have been widely utilized as platforms for enzymes immobilization in order to enhance their catalytic activities, most of immobilized enzymes exhibited reduced activities compared to free enzymes. In this study, for the first time, we used iron ions (Fe2+) and horseradish peroxidase (HRP) enzyme together to synthesize flowerlike hybrid nanostructures with greatly enhanced activity and stability and reported an explanation of the enhancements in both catalytic activity and stability. We demonstrated that Fe2+-HRP hybrid nanoflower (HNF) showed catalytic activity of ∼512% and ∼710%, respectively when stored at +4 °C and room temperature (RT = 20 °C) compared to free HRP. In addition, the HNF stored at +4 °C lost only 2.9% of its original activity within 30 days while the HNF stored at RT lost approximately 10% of its original activity. However, under the same conditions, free HRP enzymes stored at +4 °C and RT lost 68% and 91% of their activities, respectively. We claim that the drastic increases in activities of HNF are associated with to high local HRP concentration in nanoscale dimension, appropriate HRP conformation, less mass transfer limitations, and role of Fe2+ ion as an activator for HRP. Further biosensors studies based on enhanced activity and stability of HNF are currently underway.

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Keywords
HRP; Iron ion; Catalytic activity; Stability; Hybrid nanoflower
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A new generation of flowerlike horseradish peroxides as a nanobiocatalyst for superior enzymatic activity
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volumes 75–76, July–August 2015, Pages 25–29
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us