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Cell lysis induced by membrane-damaging detergent saponins from Quillaja saponaria

Paper ID Volume ID Publish Year Pages File Format Full-Text
16979 42628 2015 5 PDF Available
Title
Cell lysis induced by membrane-damaging detergent saponins from Quillaja saponaria
Abstract

•Saponin treatment was found to lead to increased yeast cell membrane permeability.•Saponin from Quillaja saponaria promotes protein and nitrogen compounds release.•Saponin may be used as an auxiliary or alternative to enzymatic methods.

This paper presents the results of a study to determine the effect of Quillaja saponaria saponins on the lysis of industrial yeast strains. Cell lysis induced by saponin from Q. saponaria combined with the plasmolysing effect of 5% NaCl for Saccharomyces cerevisiae, Kluyveromyces marxianus yeasts biomass was conducted at 50 °C for 24–48 h. Membrane permeability and integrity of the yeast cells were monitored using fluorescent techniques and concentrations of proteins, free amino nitrogen (FAN) and free amino acids in resulting lysates were analyzed. Protein release was significantly higher in the case of yeast cell lysis promoted with 0.008% Q. saponaria and 5% NaCl in comparison to plasmolysis triggered by NaCl only.

Keywords
Saponin; Yeast; Cell lysis; Yeast extract
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Cell lysis induced by membrane-damaging detergent saponins from Quillaja saponaria
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volumes 75–76, July–August 2015, Pages 44–48
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us