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Promotion of maltose fermentation at extremely low temperatures using a cryotolerant Saccharomyces cerevisiae strain immobilized on porous cellulosic material

Paper ID Volume ID Publish Year Pages File Format Full-Text
16992 42629 2014 4 PDF Available
Title
Promotion of maltose fermentation at extremely low temperatures using a cryotolerant Saccharomyces cerevisiae strain immobilized on porous cellulosic material
Abstract

•Promotion of maltose fermentation by yeast immobilized on porous cellulose.•Fermentation at 5 °C by immobilized cells was feasible.•Porous cellulose increased maltose uptake by the cells.•The activation energy of fermentation by immobilized cells was 42% lower.

Advantages in maltose fermentation at extremely low temperatures (5–10 °C) using an alcohol resistant and cryotolerant yeast strain (Saccharomyces cerevisiae AXAZ-1) immobilized on porous cellulosic material (or tubular cellulose, TC), produced by delignification of wood sawdust, are reported. Pure maltose and glucose media (80, 100 and 140 g/l) were examined as model substrates to evaluate the potential effect of TC on the rate of fermentation of maltose containing substrates. The use of TC sharply accelerated the rate of maltose fermentation compared to free cells (FC) in suspension. Fermentation at 5 °C by immobilized cells was complete, while FC were unable to ferment maltose at this temperature, in contrast to glucose, which was completely fermented. From the results of maltose and glucose fermentations at 5 and 10 °C, it was concluded that the effect of TC was higher at lower fermentation temperature and that its promotional effect on fermentation rate had to be at the step of maltose uptake. Specifically, it is suggested that the presence of TC increased maltose uptake rate by the immobilized cells due to attraction by hydrogen bonding on the TC surface and continuous pumping of maltose towards the cells. Calculation of the activation energy of maltose fermentations at 5, 10 and 15 °C showed that it was reduced by an average 42% when cells immobilized on TC were used.

Keywords
Porous cellulose; Immobilized yeast; Maltose; Low temperature fermentation; Activation energy
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Promotion of maltose fermentation at extremely low temperatures using a cryotolerant Saccharomyces cerevisiae strain immobilized on porous cellulosic material
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 66, November 2014, Pages 56–59
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us