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Immobilization of glucose oxidase on graphene and cobalt phthalocyanine composite and its application for the determination of glucose

Paper ID Volume ID Publish Year Pages File Format Full-Text
16993 42629 2014 7 PDF Available
Title
Immobilization of glucose oxidase on graphene and cobalt phthalocyanine composite and its application for the determination of glucose
Abstract

•Synthesis and characterization of GR-CoPc composite.•Direct electrochemistry of GOx at GR-CoPc composite.•Determination of glucose at GR-CoPc/GOx.•Amperometry, wide linear range.•Practicality – human Blood serum and spiked urine samples.

We described a simple and facile chemical reduction strategy for the preparation of graphene (GR)-cobalt phthalocyanine (CoPc) composite and explored it for the enzymatic determination of glucose. CoPc is an active mediator and electrocatalysts for the immobilization of GOx and determination of glucose. However, it is not stable on the electrode surface and also suffers from lack of conductivity. Here, we have employed GR as the suitable support to stabilize CoPc through simple chemical reduction method and the resulting composite has been used for the glucose biosensor application. Scanning electron microscopy, X-ray diffraction and Energy-dispersive X-ray spectroscopy studies confirmed the successful formation of composite. Direct electron transfer of glucose oxidase (GOx) was observed with well defined redox peaks at the formal potential of −0.44 V. The amount of electroactive GOx (Г) and electron transfer rate constant (ks) were calculated to be 3.77 × 10−10 mol cm−2 and 3.57 s−1, respectively. The fabricated amperometric biosensor detects glucose in wide linear concentration range from 10 μM to 14.8 mM with high sensitivity of 5.09 μA mM−1 cm−2. The sensor offered very low detection limit (LOD) of 1.6 μM. In addition, practical feasibility of the sensor has been explored in screen printing carbon electrode with accurate determination of glucose present in human blood serum and urine samples. Furthermore, the sensor exhibited appreciable stability, repeatability and reproducibility results.

Keywords
Graphene; Cobalt phthalocyanine; Glucose oxidase; Biosensor; Amperometry; Practicality
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Immobilization of glucose oxidase on graphene and cobalt phthalocyanine composite and its application for the determination of glucose
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 66, November 2014, Pages 60–66
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us