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Co-expression of d-glucose isomerase and d-psicose 3-epimerase: Development of an efficient one-step production of d-psicose

Paper ID Volume ID Publish Year Pages File Format Full-Text
16997 42630 2014 5 PDF Available
Title
Co-expression of d-glucose isomerase and d-psicose 3-epimerase: Development of an efficient one-step production of d-psicose
Abstract

•We constructed a co-expression system of d-glucose isomerase and d-psicose 3-epimerase.•We developed a one-step strategy to produce d-psicose using cheap sugar.•HFCS was high-valued by converting d-fructose to d-psicose, a zero calorie sweetener.•The amount of d-psicose was relatively high in the new products of HFCS.

d-Psicose has been attracting attention in recent years because of its alimentary activities and is used as an ingredient in a range of foods and dietary supplements. To develop a one-step enzymatic process of d-psicose production, thermoactive d-glucose isomerase and the d-psicose 3-epimerase obtained from Bacillus sp. and Ruminococcus sp., respectively, were successfully co-expressed in Escherichia coli BL21 strain. The substrate of one-step enzymatic process was d-glucose. The co-expression system exhibited maximum activity at 65 °C and pH 7.0. Mg2+ could enhance the output of d-psicose by 2.32 fold to 1.6 g/L from 10 g/L of d-glucose. When using high-fructose corn syrup (HFCS) as substrate, 135 g/L d-psicose was produced under optimum conditions. The mass ratio of d-glucose, d-fructose, and d-psicose was almost 3.0:2.7:1.0, when the reaction reached equilibrium after an 8 h incubation time. This co-expression system approaching to produce d-psicose has potential application in food and beverage products, especially softdrinks.

Keywords
Co-expression; d-Glucose isomerase; d-Psicose 3-epimerase; d-Psicose; High-fructose corn syru p(HFCS)
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Co-expression of d-glucose isomerase and d-psicose 3-epimerase: Development of an efficient one-step production of d-psicose
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volumes 64–65, October 2014, Pages 1–5
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us