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Co-immobilized glucose oxidase and β-galactosidase on bovine serum albumin coated allyl glycidyl ether (AGE)–ethylene glycol dimethacrylate (EGDM) copolymer as a biosensor for lactose determination in milk

Paper ID Volume ID Publish Year Pages File Format Full-Text
17007 42630 2014 7 PDF Available
Title
Co-immobilized glucose oxidase and β-galactosidase on bovine serum albumin coated allyl glycidyl ether (AGE)–ethylene glycol dimethacrylate (EGDM) copolymer as a biosensor for lactose determination in milk
Abstract

•Epoxy activated copolymer coated with BSA.•SH and COOH groups used for glucose oxidase and β-galactosidase immobilization.•The coupled enzyme system is used for lactose assay in milk and milk products.

Bovine serum albumin (BSA) was adsorbed on allyl glycidyl ether (AGE)–ethylene glycol dimethacrylate (EGDM) copolymer with 25% crosslink density (AGE-25) at pH 8.0 (16% w/w). The amino, thiol and carboxylic acid functional groups available on protein coated surface were utilized for covalent immobilization of glucose oxidase and β-galactosidase, both independently, and in a step-wise manner on the same matrix, with no more than 10% loss of enzyme activity during immobilization. Glutaraldehyde cross-linking after immobilization provided stable enzyme preparations. The pH-optima of the immobilized enzymes were similar to those for free enzyme but their thermal stability was vastly improved. The co-immobilized enzyme support was used as a biosensor for determination of lactose in milk with excellent reproducibility and reusability.

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Keywords
Glucose oxidase; β-Galactosidase; Bovine serum albumin; Immobilization; Lactose assay
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Co-immobilized glucose oxidase and β-galactosidase on bovine serum albumin coated allyl glycidyl ether (AGE)–ethylene glycol dimethacrylate (EGDM) copolymer as a biosensor for lactose determination in milk
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volumes 64–65, October 2014, Pages 67–73
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us